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Implementation of the TrayCell

TrayCell - Anwendungsfilm

Please click on the image to start the TrayCell video.

Quantification of Nucleic Acids

TrayCell Frau mit Pipette

To determine the nucleic acid concentration in solutions, the solutions’ absorption value is used at a wavelength of 260 nm (A260). The following function, derived from Lambert-Beer‘s Law, is applied:

Concentration [ng/μl] = Absorbance (260 nm) x Factor

(with Factor = Sample Specific Factor x Virtual Dilution Factor)

The Sample Specific Factor represents the specific absorbance for example of a sample of 50 ng/μl dsDNA that gives a reading of 1 Abs (A260), measured with an optical light path of 10 mm inside a standard cell. Due to the optical light paths of the TrayCell of 0.2 mm or 1 mm, a Virtual Dilution Factor of 50 or 10 must be taken into account additionally.

For the different types of nucleic acid solutions the average dynamic range of the absorbance relating to the concentration (ng/μl) results as follows (depending on the lightpath):

1 mm lid, (Virtual
Dilution Factor of 10)  [ng/µl] *
0,2 mm lid (Virtual
Dilution Factor 50) [ng/µl] *
Measurement
range
[ng/µl] *
dsDNA13 – 85063 – 42506 – 8500
ssDNA9 – 62946 – 31455 – 6290
ssRNA10 – 68050 – 34005 – 6800
Oligo8 – 56141 – 28054 – 5610
* typical absorbance, measured with an ordinary spectrophotometer

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